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Research Interests of Participating Faculty

Alejandro Calderón-Urrea, Biology.

The long range scientific goal of this laboratory is to understand the process of developmentally regulated programmed cell death in plants. RNA in situ hybridization is used to monitor the expression of putative plant cell death genes such as At-Dad1, and the TUNEL assay is used to monitor programmed cell death in different tissues. We also address whether or not animal cell death genes function in plant systems by using an agro-inoculation approach in tobacco plants. We monitor animal cell death gene expression in plants using RT-PCR, and also conduct enzymatic complementation assays to detect the activity of animal cell death proteins in the heterologous plant environment. Finally, this laboratory is interested in the potential use of animal cell death genes to protect plants against plant pathogenic nematodes. To this end we are generating transgenic plants expression nematodes cell death genes, which will induce the death of pathogenic nematode upon their attack of the plant. Students will be involved in all aspects of the research and will be assigned independent research projects that are overseen by a research associate and a postdoctoral scientist. We collaborate with Dr. Valerie Williamson (UC-Davis), Dr. Andrea Skantar (Nematology lab, USDA), and Lieve Gheysen (Ghent University, Belgium).

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